active rap1a detection kit Search Results


active rap1a detection kit  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc active rap1a detection kit
(A) Family tree of human superfamily of Ras-like GTPases. (B) Sequence alignment of various Ras-family GTPases. Residues mediating drug resistance to adagrasib are highlighted in light orange (rare) and orange (common). (C) Covalent modification of RalA(G23C) with compounds 1–10 (50 μM, 12 h). (D) Intact protein mass spectra of RalA(G23C)·GDP and RalA(G23C)·GDP·MRTX1257 adduct. (E) Time-dependent covalent modification of RalA(G23C) with different compounds (50 μM). (F) Differential scanning fluorimetry of RalA(G23C)·GDP and RalA(G23C)·GDP·divarasib adduct. (G) Covalent modification of <t>Rap1A(G12C)</t> with compounds 1–10 (50 μM, 12 h). (H) Intact protein mass spectra of Rap1A(G12C, L96F)·GDP and Rap1A(G12C,L96F)·GDP·divarasib adduct. (I) Time-dependent covalent modification of Rap1A(G12C) and Rap1A(G12C, L96F) with different compounds (50 μM). (J) Differential scanning fluorimetry of Rap1A(G12C, L96F)·GDP and Rap1A(G12C, L96F)·GDP·divarasib adduct. See also .
Active Rap1a Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/active rap1a detection kit/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
active rap1a detection kit - by Bioz Stars, 2026-04
95/100 stars
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rap1a human gene knockout kit  (OriGene)
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RAP1A KN2 0 Human gene knockout kit via CRISPR non homology mediated
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elisa kit rap1a rat  (G Biosciences)
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An ELISA kit for the detection of Rap1a Rat This uses Sandwich ELISA Double Antibody and has a sensitivity of 0 094ng ml
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human rap1a activation kit by crispra  (OriGene)
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RAP1A CRISPRa kit CRISPR gene activation of human RAP1A member of RAS oncogene family
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rap1a mouse gene knockout kit  (OriGene)
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Rap1a KN2 0 Mouse gene knockout kit via CRISPR non homology mediated
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rap1a human shrna plasmid kit  (OriGene)
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RAP1A Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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mouse trap1a activation kit by crispra  (OriGene)
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Trap1a CRISPRa kit CRISPR gene activation of mouse tumor rejection antigen P1A
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elisa kit rap1a human  (G Biosciences)
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An ELISA kit for the detection of RAP1A Human This uses Sandwich ELISA Double Antibody and has a sensitivity of 0 094ng ml
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trap1a mouse gene knockout kit  (OriGene)
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Trap1a KN2 0 Mouse gene knockout kit via CRISPR non homology mediated
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mouse rap1a activation kit by crispra  (OriGene)
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Rap1a CRISPRa kit CRISPR gene activation of mouse RAS related protein 1a
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Image Search Results


(A) Family tree of human superfamily of Ras-like GTPases. (B) Sequence alignment of various Ras-family GTPases. Residues mediating drug resistance to adagrasib are highlighted in light orange (rare) and orange (common). (C) Covalent modification of RalA(G23C) with compounds 1–10 (50 μM, 12 h). (D) Intact protein mass spectra of RalA(G23C)·GDP and RalA(G23C)·GDP·MRTX1257 adduct. (E) Time-dependent covalent modification of RalA(G23C) with different compounds (50 μM). (F) Differential scanning fluorimetry of RalA(G23C)·GDP and RalA(G23C)·GDP·divarasib adduct. (G) Covalent modification of Rap1A(G12C) with compounds 1–10 (50 μM, 12 h). (H) Intact protein mass spectra of Rap1A(G12C, L96F)·GDP and Rap1A(G12C,L96F)·GDP·divarasib adduct. (I) Time-dependent covalent modification of Rap1A(G12C) and Rap1A(G12C, L96F) with different compounds (50 μM). (J) Differential scanning fluorimetry of Rap1A(G12C, L96F)·GDP and Rap1A(G12C, L96F)·GDP·divarasib adduct. See also .

Journal: Cell

Article Title: Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket

doi: 10.1016/j.cell.2024.08.017

Figure Lengend Snippet: (A) Family tree of human superfamily of Ras-like GTPases. (B) Sequence alignment of various Ras-family GTPases. Residues mediating drug resistance to adagrasib are highlighted in light orange (rare) and orange (common). (C) Covalent modification of RalA(G23C) with compounds 1–10 (50 μM, 12 h). (D) Intact protein mass spectra of RalA(G23C)·GDP and RalA(G23C)·GDP·MRTX1257 adduct. (E) Time-dependent covalent modification of RalA(G23C) with different compounds (50 μM). (F) Differential scanning fluorimetry of RalA(G23C)·GDP and RalA(G23C)·GDP·divarasib adduct. (G) Covalent modification of Rap1A(G12C) with compounds 1–10 (50 μM, 12 h). (H) Intact protein mass spectra of Rap1A(G12C, L96F)·GDP and Rap1A(G12C,L96F)·GDP·divarasib adduct. (I) Time-dependent covalent modification of Rap1A(G12C) and Rap1A(G12C, L96F) with different compounds (50 μM). (J) Differential scanning fluorimetry of Rap1A(G12C, L96F)·GDP and Rap1A(G12C, L96F)·GDP·divarasib adduct. See also .

Article Snippet: Rap1A activity was measured with the Active Rap1A Detection Kit from Cell Signaling Technology (CST) 8818, according to the manufacturer’s instructions, and western blot analysis.

Techniques: Sequencing, Modification

(A) Peptides showing significant differences in HDX at any time point (>0.35 Da and >4.5%) mapped onto a homology model of RalA based on adagrasib-bound K-Ras(G12C) (PDB: 6USZ) according to the legend. (B) Intrinsic or RAPGEF5- or EDTA-mediated nucleotide exchange of BODIPY-GDP with Rap1A(G12C, L96F)·GDP and Rap1A(G12C, L96F)·GDP·divarasib adduct. (C) Immunoblot of HeLa cells transiently overexpressing EGFP-RalA(WT) and EGFP-RalA(G23C). (D) RalA activity measured by RalA G-LISA. HeLa cells were transiently transfected, treated with different concentrations of MRTX1257 for 12 h, and lysates were tested at 0.5 mg/mL. Data are presented as mean ± SEM (n = 2) and are representative of three independent experiments. (E) IP of active GTP-bound Rap1 using GST-RalGDS-RBD of HeLa cells transiently overexpressing EGFP-Rap1A(WT) and EGFP-Rap1A(G12C, L96F) and treated with different concentrations of divarasib. See also .

Journal: Cell

Article Title: Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket

doi: 10.1016/j.cell.2024.08.017

Figure Lengend Snippet: (A) Peptides showing significant differences in HDX at any time point (>0.35 Da and >4.5%) mapped onto a homology model of RalA based on adagrasib-bound K-Ras(G12C) (PDB: 6USZ) according to the legend. (B) Intrinsic or RAPGEF5- or EDTA-mediated nucleotide exchange of BODIPY-GDP with Rap1A(G12C, L96F)·GDP and Rap1A(G12C, L96F)·GDP·divarasib adduct. (C) Immunoblot of HeLa cells transiently overexpressing EGFP-RalA(WT) and EGFP-RalA(G23C). (D) RalA activity measured by RalA G-LISA. HeLa cells were transiently transfected, treated with different concentrations of MRTX1257 for 12 h, and lysates were tested at 0.5 mg/mL. Data are presented as mean ± SEM (n = 2) and are representative of three independent experiments. (E) IP of active GTP-bound Rap1 using GST-RalGDS-RBD of HeLa cells transiently overexpressing EGFP-Rap1A(WT) and EGFP-Rap1A(G12C, L96F) and treated with different concentrations of divarasib. See also .

Article Snippet: Rap1A activity was measured with the Active Rap1A Detection Kit from Cell Signaling Technology (CST) 8818, according to the manufacturer’s instructions, and western blot analysis.

Techniques: Western Blot, Activity Assay, Transfection

Journal: Cell

Article Title: Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket

doi: 10.1016/j.cell.2024.08.017

Figure Lengend Snippet:

Article Snippet: Rap1A activity was measured with the Active Rap1A Detection Kit from Cell Signaling Technology (CST) 8818, according to the manufacturer’s instructions, and western blot analysis.

Techniques: Virus, Recombinant, Protease Inhibitor, Staining, Activation Assay, BIA-KA, Mass Spectrometry, Plasmid Preparation, Software, Control, Transfection, Western Blot